First Isolation of Leishmania from Northern Thailand

A case report published in the PLoS Neglected Tropical Diseases reports the first isolation of  Leishmania from Northern Thailand. From the published abstract:


Since 1996, there have been several case reports of autochthonous visceral leishmaniasis in Thailand. Here we report a case in a 52-year-old Thai male from northern Thailand, who presented with subacute fever, huge splenomegaly and pancytopenia. Bone marrow aspiration revealed numerous amastigotes within macrophages. Isolation of Leishmania LSCM1 into culture and DNA sequence analysis (ribosomal RNA ITS-1 and large subunit of RNA polymerase II) revealed the parasites to be members of the Leishmania enriettii complex, and apparently identical to L. martiniquensis previously reported from the Caribbean island of Martinique. This is the first report of visceral leishmaniasis caused by L. martiniquensis from the region. Moreover, the majority of parasites previously identified as “L. siamensis” also appear to be L. martiniquensis.
Light micrograph of Leishmania amastigotes in bone marrow aspirate.Two infected macrophages are stained (large arrows), each with a nucleus (N) and numerous amastigotes (examples with small arrows) within the cytoplasm. The specimen was stained using Wright’s stain. The bar represents 20 µm.doi:10.1371/journal.pntd.0003339.g001

Author Summary

Visceral leishmaniasis is a potentially life threatening parasitic disease that is found in many tropical and subtropical regions of the world. We report a case of visceral leishmaniasis in northern Thailand caused by a recently described new species of Leishmania parasite, Leishmania martiniquensis. By comparing DNA sequences from this parasite with those of other isolates, we show that this parasite is more widely spread than previously thought, with several other examples in Thailand, and possibly from Europe and the USA, as well as the original location in the Caribbean island of Martinique. This parasite appears to be an emerging pathogen, which, because it causes a potentially fatal disease, needs to be investigated in detail so that its transmission can be understood and control measures can be developed.

Giemsa-stained promastigote forms from culture.A–H, examples showing morphological variation of forms observed, all at the same magnification, bar in F represents 5 µm. Procyclic-like promastigotes can be observed in A (arrows);, leptomonad-like promastigotes in B and C (arrows); nectomonad-like promastigotes in D, E and F; and rosettes and aggregates in G and H.doi:10.1371/journal.pntd.0003339.g003

Pothirat T, Tantiworawit A, Chaiwarith R, Jariyapan N, Wannasan A, et al. (2014) First Isolation of Leishmania from Northern Thailand: Case Report, Identification as Leishmania martiniquensis and Phylogenetic Position within the Leishmania enriettii Complex. PLoS Negl Trop Dis 8(12): e3339. doi:10.1371/journal.pntd.0003339 Available from: LINK.

Leishmaniasis Outbreak in ISIS Held Raqqa, Syria

Leishamaniasis, a disease caused by Leishmania donovani, and spread by the sand fly, is reportedly raging through the capital of the Caliphate held by ISIS, Raqqa, Syria. Newspaper reports contend that there may be as many as 2,500 cases of Leishmaniasis in the city, which has become the focus of military strikes by multiple nations that has targeted it as the stronghold of the ISIS.

The city of Raqqa reportedly lies in ruins and amidst massive shortage of medical personnel and drugs, the outbreak is likely to get worse. This further goes to highlight the impact that social, political and civil unrest has on health, and takes the discourse of infectious disease outbreaks and controls to domains beyond medical issues. Social, economic and political determinants are equally important in deciding the outcome of infectious diseases in today’s world.